Extended Data Figure 9: Characterization of pluripotency gene expression and epiblast morphogenesis in post-implantation human embryos.
From: Pluripotent state transitions coordinate morphogenesis in mouse and human embryos
a, Immunostaining of day 9–10 human embryos. Dotted lines indicate the epiblast and the arrow indicates the amniotic cavity. Scale bars, 50 μm and 10 μm (magnified areas). b, NANOG intensity in embryos from a. n = 4 (IVC control with lumen), 3 (IVC control without lumen) and 6 (IVC +5i/LAF) human embryos. Mann–Whitney U-test; *P = 0.0381. c, Three-dimensional reconstruction of the epiblast (based on the KLF17 staining) and the amniotic cavity (based on the PODXL staining) of embryos from Fig. 5b. d, Immunostaining of day 9–10 human embryos. n = 2 embryos per group. Scale bars, 50 μm. e, Immunostaining of day 9–10 human embryos. Scale bars, 50 μm. f, Number of apoptotic cells per embryo in embryos from e. n = 3 (IVC control) and 5 (IVC +5i/LAF) embryos per condition. Mann–Whitney U-test; *P = 0.0179.
