Extended Data Figure 5: Structural comparisons of current and previous (EMDB 6343) mouse Piezo1 structures.

For each panel, the ‘fit in map’ function of UCSF Chimera was used to align uncropped maps. In a–e, unsharpened map of C3 refinement is shown. a–c, Top (a), bottom (b) and side (c) views of current Piezo1 (green) and EMDB accession EMD-6343 (grey) structurally aligned. d, Expanded top view of the cap domain to illustrate an approximately 15° rotation of the cap between the two maps. e, Expanded sliced top view of pore-proximal transmembrane region. Subtle conformational rearrangements are present between the current and previous structures in the inner and outer helices. f, g, Top and side views of EMDB accession EMD-6343 (grey), blade class 1 (blue), and blade class 2 (orange). The trajectories of the propeller blades are broadly similar across the maps in both the membrane-parallel and membrane-normal directions.