Extended Data Figure 6: Structural features of Piezo repeats.
From: Structure of the mechanically activated ion channel Piezo1
a, Top view of Piezo1 depicted as cartoon, with amphipathic helices from Piezo repeats A, B and C shown in red. Extracellular cap domain is omitted for clarity. b–d, Ribbon models of amphipathic helices from Piezo repeat A (b), Piezo repeat B (c), and Piezo repeat C (d). Cα positions are shown as spheres and hydrophobic amino acids are coloured grey, basic amino acids are coloured blue, acidic amino acids are coloured red, and polar uncharged amino acids are coloured yellow. e, Cartoon model of a portion of the Piezo1 propeller blade, with Piezo repeats coloured separately. A strong density peak putatively representing a lipid head group is shown as a pink mesh. f, Expanded view of the putative lipid binding site sandwiched between Piezo repeats B and C (helices B4 and C1). Electron density (blue mesh) is superimposed onto the molecular model, and the putative lipid head group is shown as pink mesh. Side chains contributing to the binding pocket are shown as yellow sticks and labelled.
