Extended Data Figure 1: Purification and electron microscopy analysis of Piezo1.
From: Structure of the mechanically activated ion channel Piezo1
a, Preparative gel filtration chromatogram of mouse Piezo1 after affinity purification and proteolytic removal of the GST tag. b, SDS–PAGE analysis of Piezo1 following affinity purification (pre-SEC) and after subsequent size-exclusion chromatography (SEC) steps (SEC fractions). Purifications of Piezo1 have been repeated more than three times with similar results. c, Aligned micrograph of purified Piezo1 embedded in a thin layer of vitrified ice. Scale bar, 100?nm. d, Representative 2D classes of Piezo1 showing different particle orientations.
