Extended Data Figure 3: Asymmetry of Piezo1 and blade-focused classification and refinement.
From: Structure of the mechanically activated ion channel Piezo1
a, Duplicate unsharpened maps of Piezo1 refined without symmetry imposed, with the blue map rotated approximately 120° around the central axis of pseudosymmetry relative to the pink map, then superimposed with the ‘fit in map’ function of UCSF Chimera. The left panel shows top view, while the right panel shows a horizontal slice through the transmembrane region. b, Flow chart of propeller blade-focused classification and refinement procedure. c, Local resolution maps calculated by the locres program in RELION 2.0. Colour key for local resolution (in Å) is shown and is the same as Extended Data Fig. 2b. d, Angular distributions of the particles after the final step of refinement in RELION. The radius of the sphere is proportional to the number of particles with a given orientation. e, FSC plots calculated using relion_postprocess for unmasked maps and maps with a soft mask applied to remove the contribution of scattered detergent density.
