Extended Data Figure 2: Catalytically deficient SHMT2 constructs.
From: Mitochondrial translation requires folate-dependent tRNA methylation
a, Mapping of mutated amino acid residues on human SHMT1 (PDB code 1BJ481) using iCn3D and alignment of E. coli serine hydroxymethyltransferase (GLYA), H. sapiens mitochondrial serine hydroxymethyltransferase 2 (GLYM) and cytosolic serine hydroxymethyltransferase 1 (GLYC). Positions for GLYM are given with reference to GenBank NM_005412.5. b, Sanger sequencing traces of mutant constructs. c, Immunoblot for mitochondrial complex I levels (NDUFS4) in cell lines re-expressing catalytically deficient forms of SHMT2.
