Extended Data Figure 6: Generation and characterization of Ten3flox.
From: Teneurin-3 controls topographic circuit assembly in the hippocampus
a, Design of Ten3fl. Top: region of chromosome 8 containing Ten3 exon 4, which is 239 base pairs long and encodes 19 of the 21 amino acids in the transmembrane domain. Guide RNA (gRNA) targets shown in red (see key at right). Line 2: targeting construct with loxP sites inserted 5′ and 3′ of exon 4. Neo includes a PGK promoter driving the resistance gene. Line 3: genomic region after homology-directed repair. Bottom: deletion of exon 4 after Cre-mediated recombination between loxP sites. Neo was removed by FLP-mediated recombination in some of the mice used in Figs 3 and 4. In addition to deleting exon 4, the reading frame 3′ to exon 4 is frame-shifted with respect to the reading frame 5′ to exon 4. b–d, Cre (green) and Ten3 (magenta) protein expression in P10 horizontal sections from Ten3fl/+ (b), Ten3Het (c), and Ten3cre/fl (d) mice. Ten3 staining is absent in Ten3cre/fl mice. White dotted lines highlight proximal and distal borders of CA1 and subiculum. Scale bars, 200 μm.
