Extended Data Figure 9: Latrophilin-3 and Ten3 aggregation assay.
From: Teneurin-3 controls topographic circuit assembly in the hippocampus
a, Images from aggregation assay with cells co-transfected with latrophilin-3 (Lphn3) and mCherry (magenta) mixed with cells co-transfected with GFP and empty vector (left), A0B0 isoform of Ten3 (middle), or A1B1 isoform of Ten3 (right). Scale bar, 200 μm, applies to all images. b, Quantification of aggregate sizes pooled from three biological replicates. Dashed red line shows cutoff at 600 μm2, the size of a large GFP+ cell from the control images. Asterisks denote significance from Dunn’s multiple comparisons test after a Kruskal–Wallis test, comparing all conditions with the Lphn3 and control mix, Lphn3 + control: n = 32 particles above threshold; Lphn3 + A0B0: n = 172; Lphn3 + A1B1: n = 159. NS, not significant; ****P ≤ 0.0001, multiplicity-adjusted P values.
