Extended Data Figure 10: Aggregation assays for cells expressing different Ten3 splicing isoforms.
From: Teneurin-3 controls topographic circuit assembly in the hippocampus
a, Cell aggregation assay with combinations of K562 cells expressing the A1B1, A2B1, or A3B1 Ten3 isoform along with GFP or mCherry. Scale bar in bottom right panel, 200 μm, applies to all images. b, Quantification of aggregates observed in three biological replicates of the aggregation experiment in a. At least 100 aggregates were counted across the three replicates in each of the 10 mixing conditions, except for the GFP-alone and mCherry-alone controls, where no aggregates were observed. One hundred per cent of aggregates were mixed in combinations where both cell populations expressed a Ten3 isoform. No mixed aggregates were observed in combinations of Ten3-expressing cells with cells expressing mCherry alone, confirming that the aggregation is Ten3-dependent and not due to an endogenously expressed interaction partner. Fractions in parentheses indicate aggregates of a particular type out of all aggregates counted in that condition.
