Extended Data Figure 4: Generation and characterization of Ten3cre.
From: Teneurin-3 controls topographic circuit assembly in the hippocampus
a, Design of Ten3cre. Top: region of chromosome 8 containing Ten3 exon 1, which contains the start codon (ATG). Middle: targeting construct with cre open reading frame inserted directly after the Ten3 start codon. cre is followed by a synthetic intron, woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and bovine growth hormone polyadenylation sequence (bGH pA) (see key on right). Neo includes a phosphoglycerate kinase (PGK) promoter driving the resistance gene. Bottom: genomic region after homologous recombination. The endogenous exon 1 sequence after the start codon is replaced with cre. Neo was not removed by flippase (FLP)-mediated recombination in the mice used in Fig. 2. b–d, Cre and Ten3 protein expression in P10 horizontal sections from Ten3cre mice. White dotted lines highlight proximal and distal borders of CA1 and subiculum. b, c, Cre expression (green) mimics the distribution of Ten3 expression (magenta) in Ten3Het mice. d, In Ten3cre/cre mice, Ten3 immunostaining is absent. Scale bars, 200 μm.
