Extended Data Figure 4: Additional analysis of calcium imaging from food neophobia studies.
a–c, Change in calcium fluorescence activity in response to a novel, high-fat diet (HFD) pellet. Test trials were conducted on consecutive days. Line graphs are means ± s.e.m. (shaded region) for raw ΔF/F values and plots show normalized ΔF/F values for neurons (rows) of all experimental mice. d–f, Average calcium activity 5 s before and 5 s after presentation of HFD pellet during three test trials. g–i, Average calcium activity 5 s before and 5 s after taking a bite from the HFD pellet. Bar graphs are means ± s.e.m. Test trial 1, n = 5 mice, 247 neurons; trial 2, n = 5 mice, 244 neurons; trial 3, n = 5 mice, 254 neurons. Βaseline was 60-s period before HFD pellet was presented. ***P < 0.001; d–i, paired Student’s t-test (two-tailed). Related to Fig. 4. For statistical analysis, see Supplementary Information.
