Extended Data Figure 5: CGRPPBN neurons are activated during exposure to a novel marble.
a–c, Change in calcium activity in response to a marble being placed in home cage. Line graphs are means ± s.e.m. (shaded region) for raw ΔF/F values and plots show normalized ΔF/F values for neurons (rows) of all experimental mice. d–f, Average calcium activity 5 s before and 10 s after placing a marble in the cage. Graphs are means ± s.e.m. Test trial 1, n = 4 mice, 220 neurons; trial 2, n = 4 mice, 218 neurons; trial 3, n = 4 mice, 222 neurons. Βaseline was 60-s period before marble was presented. ***P < 0.001; d–f, paired Student’s t-test (two-tailed). Related to Fig. 4. See also Supplementary Video 4. For statistical analysis, see Supplementary Information.
