Extended Data Figure 6: Additional calcium imaging analysis from fear recall studies.
a, b, Average calcium fluorescence activity during 10-s tone presentations in fear-conditioned (a) and sham-conditioned (b) mice during tone trials 1, 15, and 30. Relative fluorescence was calculated from a 5-s baseline period before tone presentation. Graphs are mean ± s.e.m.; fear-conditioned mice, n = 4 mice, 214 neurons; sham-conditioned mice, n = 4 mice, 169 neurons. c, Contextual fear conditioning paradigm in which mice were placed in a shock chamber before (Pre 1 and Pre 2) and after (Post 1 and Post 2) footshock conditioning. d, Calcium activity of individual neurons (horizontal lines are means) during a 5-min exposure to the shock chamber before and after fear conditioning (Pre 1, n = 3 mice, 120 neurons; Pre 2, n = 3 mice, 125 neurons; Post 1, n = 3 mice, 124 neurons; Post 2, n = 3 mice, 121 neurons). Relative fluorescence was normalized to a 2-min baseline recording period. e, Calcium activity of individual neurons (horizontal lines are means) during freezing and non-freezing behaviour after conditioning. f, Percentage of time spent freezing during exposure to the shock chamber after conditioning. Data points represent individual mice (n = 3). g, Representative individual neuron traces showing calcium activity while in home cage versus shock chamber after contextual fear conditioning. The dotted line denotes when the shock chamber recording began. The shaded regions annotate when the mouse was freezing. Dissimilar letters above columns indicate statistical differences between trials. ***P < 0.001; a, d, e, One-way repeated measures ANOVA, Tukey’s post-hoc. Related to Fig. 5. For statistical analysis, see Supplementary Information.
