Extended Data Figure 1: CGRPPBN neurons are activated by facial pain and electrical shock.
a, b, Percentage of cells activated by tail pinch and placing a warm metal rod on the lip of anaesthetized mice (n = 6 mice, 397 neurons). c, Representative calcium traces of the same individual CGRP neurons during baseline and 2 h after LPS injection in awake mice. d, Average calcium fluorescence activity 1 s before (Base) and during a footshock (0.5 mA, 0.5 s duration; n = 5 mice, 317 neurons); bar graph is mean ± s.e.m.. e, Calcium activity time course of CGRP neurons (n = 3 mice, 158 neurons) and oxytocin receptor neurons (n = 3 mice, 65 neurons) in response to electrical tail shock (2 mA, 2 s duration) in anaesthetized mice; baseline was 2-s period before shock. AAV-DIO-GCaMP6m was injected into the lateral PBN of CalcaCre/+ and OxtrCre/+ mice. For imaging oxytocin receptor neurons, the lens was placed over the dorsal lateral PBN, in contrast to external lateral PBN targeting for CGRP neurons. Line graph is mean ± s.e.m. (shaded region). ***P < 0.001, paired Student’s t-test (two-tailed). Related to Fig. 1. For statistical analysis, see Supplementary Information.
