Extended Data Figure 2: CGRPPBN neurons encode stimulus intensity.
a, Plot shows calcium fluorescence activity of neurons in an anaesthetized mouse following placement of the tail in a water bath ranging from 40 °C to 52 °C (n = 56 neurons). b, Area under the curve of calcium activity following placement of tail in 40–52 °C water bath (n = 5 mice, 286 neurons). c, Percentage of cells activated by placement of tail in 56 or 60 °C water in anaesthetized mice; data points are individual mice (n = 4 mice) and line represents mean. d, Time course and peak neuronal responses to tail being placed in 56 or 60 °C water (n = 4 mice, 111 neurons). Baseline was 10-s period before placing tail in water. e, Area under the curve of calcium activity following placement of tail in water bath. f, Time course and peak neuronal responses to tail receiving graded intensities of electrical current (2–6 mA, 2 s duration; n = 3 mice, 105 neurons) in anaesthetized mice. Baseline was 2-s period before shock. g, Time course and peak calcium activity of neurons in an awake mouse (46 neurons) during exposure to a hotplate (40–56 °C, 20-s duration). Baseline was 2-min period in home cage before the hotplate test. h, Area under the curve of calcium activity following placement of awake mice on the hotplate (n = 3 mice, 126 neurons). Bar graphs are mean ± s.e.m.; line graphs are mean ± s.e.m. (shaded region). ***P < 0.001; dissimilar letters above columns indicate statistical differences between treatments. b, h, One-way repeated measures ANOVA, Tukey’s post-hoc; e, paired Student’s t-test (two-tailed). Related to Fig. 1. For statistical analysis, see Supplementary Information.
