Extended Data Figure 3: Additional analysis of calcium imaging from fast–refeed study.
a, Average calcium fluorescence activity 5 s before and 5 s after presentation of standard chow pellet (n = 5 mice, 282 neurons); mean ± s.e.m.. b, Calcium activity relative to taking a bite (n = 5 mice, 286 neurons). c, d, Plot showing calcium activity (normalized ΔF/F) relative to placement of chow in cage (c) and relative to bite (d). Plots show neurons (rows) from all experimental mice (n = 5 mice, 282 neurons). e, f, Representative calcium traces extracted using CNMF analysis, relative to chow availability (e) and taking a bite (f). Βaseline was 60-s period before chow availability for all data shown. ***P < 0.001; dissimilar letters above columns indicate statistical differences between time points; a, paired Student’s t-test (two-tailed); c, one-way repeated measures ANOVA, Tukey’s post-hoc. Related to Fig. 3. For statistical analysis, see Supplementary Information.
