Extended Data Figure 10: Subclasses of interneurons and signal pathways regulating neurogenesis.
From: A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex
a, Heat map of illustrated subclasses of interneurons. The bar chart on the top shows the gestational week. b, The expression of known markers of interneurons mapped to the Monocle analysis (Fig. 4a) shows the pseudo-time course of interneurons during development (grey, no expression; yellow–red, relative expression). c, In situ hybridization of TTF1 shows the postion of interneuron progenitor cells in PFC at GW10. Scale bar, 100 μm. n = 3 independent replicates. d, Pattern of marker gene expression in interneuron progenitor cells mapped onto the cell cycle plot for all interneurons. Interneurons with high expression of TTF1, LHX6, DLX1 and DLX2 exhibit low expression of cell cycle genes. e, Quantification of specific markers for interneuron progenitor cells and interneurons of PFC at GW7 by reverse transcription–PCR analysis. n = 3 independent replicates. f, In situ hybridization of SST in PFC at GW10. Scale bar, 100 μm. g, Immunostaining of SST in PFC at GW10. Scale bar, 100 μm. n = 3 independent replicates. h, GSEA enrichment plot of the KEGG neurotrophin signalling pathway. n = 663 excitatory neurons; n = 485 interneurons. i, Mean expression of Notch signalling pathway genes in NPCs, excitatory neurons and interneurons at different stages of development. The bar chart at the top represents the ratio of expressed genes to all genes in the Notch pathway.
