Extended Data Figure 5: Immunostaining of neural progenitor cells and microglia in the developing PFC.
From: A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex
a, Immunostaining for known markers (PAX6 and SOX2 for NPCs, NEUROD2 for excitatory neurons) at GW8, GW12, GW16, GW19 and GW23, showing the position of NPCs and excitatory neurons. Scale bars, 100 μm (left) and 25 μm (right). n = 3 for GW 19 and GW23, n = 4 for GW8, GW12 and GW16. b, Bar charts showing the proportion of PAX6+SOX2+ cells in SOX2+ cells in the ventricular zone, iSVZ and oSVZ in the PFC at GW8, GW12, GW16, GW19 and GW23 relative to the images in a. Data are mean ± s.e.m. c, Co-staining for AIF1 and CD45 to label microglia in the PFC at GW16. d, Immunostaining for the microglia marker CD45 in the PFC at GW23. Scale bar, 100 μm. n = 3 independent replicates per gestational week.
