Extended Data Figure 11: Position of the FG-repeat anchor points and heat mapping of the FG repeats.
From: Integrative structure and functional anatomy of a nuclear pore complex
a, Three views of the complete structure of the NPC are shown with major structural features (coloured as in Fig. 4 and Supplementary Table 2) and a snapshot of modelled FG-repeat regions (indicated in green). For each Nup, the localization probability density of the ensemble of structures is shown with a representative structure from the ensemble embedded within it. See also Supplementary Videos 1, 2, 3. Scale bar, 200?Å. b, Positions of FG-repeat anchor points within the ensemble of solutions are depicted as green surfaces; the Nups to which they belong are labelled in the centre image. Left, side view of three spokes; centre, side view of one spoke; right, top view of three spokes. Scale bar, 100?Å. c, Heat mapping of the type of FG-repeat region of each FG Nup (FXFG/FG type, red; GLFG type, blue), showing partitioning of the FG types to different regions of the central transporter. Identity of mapped Nups is shown in the diagram on the right. Scale bar.100?Å. d, Heat mapping of the effect on NPC permeability of the truncation of an FG repeat in each FG Nup, relative to the wild-type strain (p/pWT); the severity of the permeability defect is indicated in increasing intensity of shades of blue from minor defect (light green) to severe defect (dark blue), thereby defining the FG repeats that are most important in maintaining the passive permeability barrier. Identities of mapped Nups are shown in the diagram on the right. Scale bar, 100?Å.
