Supplementary Figure 10: Base-skipping CRISPR mutants mediate efficient on-target cleavage. | Nature Biotechnology

Supplementary Figure 10: Base-skipping CRISPR mutants mediate efficient on-target cleavage.

From: Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

Supplementary Figure 10

HEK293T cells were transfected with the Cas9 expression plasmids with (a) WAS CR-4 and (b) WAS CR-5 as indicated, and the genomic DNA harvested 48 h later was assessed using the Surveyor assay (n=3). Efficient target cleavage was observed in some mutants with one-base skipping. Arrows indicate the positions of the expected fragments. UD, undetectable.

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