Supplementary Figure 1: Indel formation by CRISPRs and TALENs targeting the WAS and TAT genes. | Nature Biotechnology

Supplementary Figure 1: Indel formation by CRISPRs and TALENs targeting the WAS and TAT genes.

From: Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

Supplementary Figure 1

(a) Sequences of the CRISPRs and TALENs targeted regions in the WAS and TAT genes. Bases in grey indicate the PAM sequence. (b) Surveyor assay to detect CRISPR-mediated indel formation in the WAS and TAT genes (n=3). (c) Surveyor assay to detect TALEN-mediated indel formation in the WAS,TAT, ZRSR2 and miR-9-2 genes. HEK293T cells were transfected with the indicated CRISPR/Cas9 or TALEN pairs. Cells transfected with the GFP gene served as the negative control. Genomic DNA extracted 48 h later was assessed for target cleavage with the Surveyor assay (n=3). Results from duplicated transfection are shown. Efficiency of indel formation is shown below each lane. Arrows indicate the positions of the expected fragments.

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