Supplementary Figure 2: Genomic mapping of the IDLV-puro integration sites.

(a) Schematic display of mapping the IDLV-puro integration sites. Primer used for nrLAM-PCR is located in the U5 region of the lentiviral long terminal repeat (LTR) and is indicated with a half-arrowhead. The linear PCR products are ligated to a linker, followed by nested PCR to yield sufficient DNA for deep sequencing. (b) Representative photographs of IDLV-transduced colonies after puromycin selection. HEK293T cells were transfected with either CRISPR or TALEN, followed by tranduction twice with IDLV-puro. Puromycin selection was initiated two days after transduction. HEK293T cells transduced with IDLV-puro without CRISPR or TALEN served as the negative control. Expression of CRISPR or TALEN led to a 2-3 fold increase in the puromycin-resistant colonies (n=2).