Supplementary Figure 5: Confirmation of IDLV mapped off-target sites with the Surveyor assay. | Nature Biotechnology

Supplementary Figure 5: Confirmation of IDLV mapped off-target sites with the Surveyor assay.

From: Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

Supplementary Figure 5

HEK293T cells were transfected with the expression plasmids for Cas9 and (a) WAS CR-4, (b) WAS CR-5, (c) TAT CR-1. Cells transfected with the expression plasmids for Cas9 and GFP genes served as the negative control. Genomic DNA isolated 48 h later was subjected to the Surveyor assay (n=3), and percent of indel formation was determined. Results from duplicated transfection are shown. Arrows indicate the positions of the expected fragments. The gene near the CRISPR/Cas9 cleavage site is indicated on top of each gel. UD, undetectable.

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