Supplementary Figure 6: Confirmation of the on- and off-target sites of CRISPR/Cas9 in HT1080 cells. | Nature Biotechnology

Supplementary Figure 6: Confirmation of the on- and off-target sites of CRISPR/Cas9 in HT1080 cells.

From: Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

Supplementary Figure 6

Human fibrosarcoma HT1080 cells were transfected with the expression plasmids for Cas9 and the indicated CRISPR. The genomic DNA extracted 48 h later was subjected to the Surveyor assay (n=3). (a) On-target cleavage by the indicated CRISPR/Cas9. (b) Off-target cleavage by the indicated CRISPR/Cas9. Cells transfected with the GFP/Cas9 genes served as the negative control. The gene near the off-target site is indicated on top of each gel. Arrows indicate the positions of the expected fragments. UD, undetectable.

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