Supplementary Figure 8: Deep-sequencing analysis of CRISPR/Cas9-mediated cleavage of in silico predicted POT sites.

PCR primers were designed for each POT site with the expected cleavage site centrally located in the PCR product. The genomic sequence is shown in the first row and the red dash lines in this row indicate the space created to accommodate base insertion generated by NHEJ in this genomic region. The sequences of indel determined by deep sequencing are shown below each genomic sequence. Black dash lines indicate base deletion and red nucleotides indicate base insertion. Underlined bases indicate the off-target recognition sequence; bases labeled in grey denote the PAM sequence; arrows indicate the CRISPR/Cas9 cleavage site. The sequences with greater than 10 reads as determined by deep sequencing were calculated and only the top 20 reads were shown.