Supplementary Figure 9: Populations mask variation and asynchrony.

Pools of 20 cells were simulated from single cell data and projected onto the principal component analysis for all 3,934 cells. Each plot shows one embryonic stage and the simulated pools of 20 cells for that population in black (normalized in the same way as single cells). Bottom right hand plot shows the head fold stage and simulated pools as well as actual pools of 20 cells sorted from embryos (pink).