Supplementary Figure 4: RNA sequencing of populations of 50 cells.

Schematic of early embryonic development of the mesodermal lineage. The cardiac, smooth muscle, endothelial and blood lineages all diverge from Flk1+ mesoderm, while somatic muscle diverges earlier. (a-f) Populations of 50 cells were sorted from 5 embryos each at PS, NP and HF stages. FPKM values for three key genes are shown for each of the lineage decisions in the schematic. Points are the mean and s.d. of the 5 replicates.