Supplementary Figure 9: Expression of the X-linked CMV-GFP reporter and X-linked Uba1 gene in female TTFs and derivative iNSCs
From: Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage
(A) Flow cytometry analysis of OKSM-iNSC clones generated either from sorted XaXiGFP (top) or XaGFPXi tail tip fibroblasts (TTFs) (bottom). Note that some iNSC clones partially lose CMV-GFP reporter expression after prolonged culture, regardless of their origin from XaXiGFP or XaGFPXi TTFs. The PE-Cy7 channel was used to control for autofluorescence. (B) Sorted XaXiGFP TTFs do not reactivate the CMV-GFP reporter located on the silent X chromosome after 2 weeks of culture in NSC media. A positive control for GFP expression is shown on the right. The APC channel was used to control for autofluorescence. (C) Allele-specific quantitative PCR analysis of the X-linked gene Uba1. Primers distinguishing between the maternal mus musculus musculus (left) and the paternal mus musculus castaneous (right) alleles of the Uba1 gene were used. Sorted GFP+ and GFP- TTFs served as controls.
