Supplementary Figure 13: Characterization of BKSM-iPSCs
From: Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage
(A) Representative images of iPSCs generated with the BKSM cassette from Sox2-GFP MEFs (BKSM-iPSCs). (B) Expression of key pluripotency genes in BKSM-iPSCs based on microarray analysis. (C) Scatter plot analysis showing linear regression coefficient (R2) of global gene expression between MEFs and BKSM-iPSCs, and mouse ESCs and BKSM-iPSCs. (D) Teratoma sections from a BKSM-iPSC clone showing cell types representative of the three germ layers. (E) Activation of Sox2-GFP expression in the brain of a neonatal chimera generated from BKSM-iPSCs (derived from Sox2-GFP MEFs) (left panel) relative to a non-chimeric control (right panel).
