Supplementary Figure 2: Molecular characterization of iPSCs generated in NSC media and propagated in ESC media
From: Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage
(A) Graph showing quantification of Oct4+ and Nanog+ iPSC-like colonies generated in NSC media. For each replicate, 3x104 cells were used (n=3 independent replicates; error bars, s.e.m. *P<0.05). (B) Representative immunofluorescence images showing co-localization of Oct4+ and Nanog+ in colonies emerging in NSC media. (C) Morphology of NSC media-derived iPSCs (upper panel) and alkaline phosphatase (AP) staining for an expanded iPSC clone (lower panel). (D) Representative images of immunofluorescence staining for Nanog in NSC media-derived iPSC clones #1-3. (E) Expression of pluripotency-associated surface markers in NSC media-derived iPSCs by live-staining immunofluorescence analysis. (F) Flow cytometry analysis of surface markers characteristic of nascent iPSCs (loss of Thy1, gain of Epcam) in rep-MEFs 6 days after dox administration in conventional NSC media. (G) Gene expression levels of Epcam in the indicated cell types by microarray gene expression analysis. Note that Epcam is not expressed in brain-NSCs or OKSM-iNSCs.
