Supplementary Figure 5: Molecular characterization of iPSCs generated from Oct4-CreER; R26-lsl-EYFP MEFs in NSC media and propagated in ESC media
From: Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage
(A) iPSC clones remain EYFP- in the absence of 4-OHT (left panels) while they become EYFP+ in the presence of 4-OHT (right panel). (B) Representative immunofluorescence stains for Nanog and Oct4 in NSC media-derived iPSC clones generated from Oct4-CreER; R26-lsl-EYFP MEFs. (C) Flow cytometry analysis for EYFP expression in MEFs derived from chimeric embryos generated from EYFP+ iPSCs clone #6 (generated in conventional NSC media). Shown are MEF preparations established from 2 different E13.5 embryos and analyzed at passage 2. Non-chimeric EYFP- MEFs served as a negative control. The PE-Cy7 channel was used to control for autofluorescence.
