Supplementary Figure 1: The mass accuracy of fragment ions is important for peptide recovery in wide-tolerance searches. | Nature Biotechnology

Supplementary Figure 1: The mass accuracy of fragment ions is important for peptide recovery in wide-tolerance searches.

From: A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides

Supplementary Figure 1

The same data as in Figure 1B was searched with varying fragment ion tolerances (FIT). Peaks in MS/MS spectra were thus required to match with differing tolerances. A 1.0 Da FIT is typically used to search spectra collected at lower resolution. For the ±500-Da precursor ion search, only 43.9% of peptides were recovered for a FIT of 1.0 Da. In contrast, the ±500-Da precursor ion search using FIT of 0.01 recovered 85.5% of peptides.

Back to article page