Supplementary Figure 7: G4-seq analysis obtained using PhenDC3 (1 μM) as a G4-stabilizing ligand.

a) % mismatches for the control sequences in the same sequencing experiment with Na⁺ sequencing buffers for the first read (Read-1; left) followed by the addition of the small-molecule PhenDC3 in Na⁺ throughout the second read (Read-2; right). b) % OQs detected in both K⁺ and PDS G4-Seq experiments (Left), K⁺ and PhenDC3 (Middle), PDS and PhenDC3 (Right). 85% of all OQs detected in PDS were also observed with PhenDC3. c) % PQs detected in both K⁺ and PDS G4-Seq experiments (Left), K⁺ and PhenDC3 (Middle), PDS and PhenDC3 (Right). 93% of all PQs detected in PDS were also observed with PhenDC3. d) % predicted G4s (PQs) among all the detected G4s (OQs) in different G4-Seq experiments. PhenDC3 and PDS show similar capability to detect G4s genome-wide. e) Summary of the comparison between different G4-Seq experiments.