Supplementary Figure 4: Aggregation of Drop-ChIP data from 200 single cells yields a high quality profile that recapitulates bulk ChIP-seq.
From: Single-cell ChIP-seq reveals cell subpopulations defined by chromatin state
A) Chromatin maps generated by aggregating Drop-ChIP data from 200 single cells (‘200’) closely match conventional ChIP-seq data (‘Bulk’). Tracks depict H3K4me3 or H3K4me2 in ES cells, MEFs or EML cells for intervals on Chr6: 125,140,000-125180000 and Chr17: 35,486,000-35,526,000. Scatter plots (right) compare aggregate profile signals against conventional ChIP-seq signals in non-overlapping 5kb windows in chromosome 19 (genome-wide correlations between respective datasets are indicated, top right plot is identical to Supplementary Figure 3E). B) Receiver operating characteristic (ROC) curve plots the fraction of true peaks vs. the fraction of false peaks called from H3K4me3 chromatin profiles derived by aggregating the indicated numbers of ES cell profiles. Area under the curve (AUC) for aggregates of different sizes is plotted in the inset. Excellent recovery of bulk peaks (AUC>0.9) is attained for 500 single-cells or more.
