Supplementary Figure 6: Single-cell chromatin profiles de-convolve mixed cell populations.
From: Single-cell ChIP-seq reveals cell subpopulations defined by chromatin state
A) A mixture of ES cells and MEFs was applied to the microfluidics device and subjected to Drop-ChIP for H3K4me3. Divisive hierarchical clustering was then applied to the single-cell profiles as described in the methods. The resulting hierarchal tree reveals two major clusters, whose aggregate profiles closely match bulk H3K4me3 profiles for ES cells and MEFs, respectively (shown for region on Chromosome 17). B) Re-clustering of these cells together with single-cell H3K4me3 profiles from pre-labeled ES cells and MEFs confirms that the original completely unbiased clustering distinguished ES cells from MEFs with >95% specificity and sensitivity.
