Supplementary Figure 6: Gating strategies for flow cytometry analysis.
From: GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell interactions
(a) Representative gating strategy of the spleen of B10D2 mice after injection of either LV.GFP or LV.YFP and adoptive transfer of either control or Jedi T cells. This gating strategy was used in Figure 1c and 1e. (b) Representative gating strategy of the in vitro cultures of BM-DCs and T cells to determine T cell proliferation. This gating strategy was followed in Supplementary Figure 2c. (c) Representative gating strategy of the brain from CX3CR1-GFP mice after adoptive transfer of either control or Jedi T cells. This gating strategy was used in Figure 2. (d) Representative gating strategy of the blood from FoxP3-GFP mice after adoptive transfer of either RFP+ control or RFP+ Jedi T cells. This gating strategy was used in Figure 3b and c. (e) Representative gating strategy of the heart from Hcn4-GFP mice after adoptive transfer of either control or Jedi T cells. This gating strategy was used in Figure 4d.
