Supplementary Figure 2: Jedi T cells specifically recognize EGFP
From: GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell interactions
(a) Sequence of the rearranged alpha and beta chains in the Jedi mice. (b) Comparison of the amino acid sequence between similar fluorescent proteins, namely Yellow Fluorescent Protein (eYFP), mCitrine, Green Fluorescent Protein (eGFP) and mCerulean. Highlighted in bold is the immune epitope of eGFP. (c) Bone marrow cells were transduced with a lentivirus expressing EYFP or EGFP and cultured in the presence GM-CSF to generate dendritic cells (BMDCs). Isolated Jedi or B10D2 CD8+ T cells were stained with Violet Cell Proliferation dye and added to BMDCs. EGFP-specific cells were identified by H-2Kd-GFP200-208 pentamer staining. Graph present the mean±s.d. of the frequency of cells that have proliferated (n=3 wells/group). ***P<0.001 vs each group.
