Supplementary Figure 3: Jedi T cells cross the blood brain barrier and kill EGFP-expressing microglia.
From: GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell interactions
(a) Blood from bone marrow transplanted CX3CR1-EGFP mice was obtained 10 weeks after the transplant to confirm bone marrow engraftment prior to the transfer of the Jedi T cells. (b) Representative flow cytometry plots showing EGFP expression in the bone marrow (BM) of BM transplanted CX3CR1-EGFP mice 1 week after transferring Jedi T cells. (c) Representative flow cytometry plots showing EGFP expression in the lymph nodes (LN) of BM transplanted CX3CR1-EGFP mice 1 week after transferring Jedi T cells. (d) Representative images of the brain showing EGFP expression (signifying microglia) on tissue sections in different regions of the brain (20x magnification, bar represents 50 µm). (e) Representative flow cytometry plots showing CD45.1 Ctrl or Jedi T cells in the brain of BM transplanted CX3CR1-EGFP mice 1 week after transferring the indicated type of T cells. Graphs show the percentage of CD45.1+ cells compared to total CD45+ cells and the total number of CD45.1+ cells in the brain (n=3 mice/group). ***P<0.001 vs Control-treated. (f) Representative images of the brain showing EGFP expression (signifying microglia) and T cells (by CD3 staining) on tissue sections (20x magnification, bar represents 50 µm).
