Supplementary Figure 5: Screening for growth factors and small molecules to induce renal vesicles | Nature Biotechnology

Supplementary Figure 5: Screening for growth factors and small molecules to induce renal vesicles

From: Nephron organoids derived from human pluripotent stem cells model kidney development and injury

Supplementary Figure 5

(a, b) The tested protocols for renal vesicle induction. (c) Immunocytochemistry for SIX2 and LHX1 in structures on day 14 of differentiation. Transient treatment with CHIR 3 μM from day 9 to 11, in combination with FGF9 10 ng/ml from day 7 to 14, increased the number of LHX1+ cells and suppressed SIX2 expression, suggesting mesenchymal epithelial transition. Scale bars: 50 μm. REGM: renal cell growth medium (Lonza, #CC-3190).

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