Supplementary Figure 4: Correlation of action potential (AP) parameters and mRNA expression for synaptic proteins, receptor subunits. | Nature Biotechnology

Supplementary Figure 4: Correlation of action potential (AP) parameters and mRNA expression for synaptic proteins, receptor subunits.

From: Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes

Supplementary Figure 4

(a) Heat map of correlation coefficients (scaled, color-coded and filtered to <−0.4 or >0.4 and from >5 cells) of mRNA counts and characteristic electrophysiological properties of dual-labeled interneurons. Open rectangles denote significant examples in our predictive matrix, which are shown in (d,e). (b) Color matrix marks the relationship of individual parameters and particular time-locked phases of a single action AP or AP waveforms (in c). (d,e) Correlation between electrophysiology parameters and gene expression of Syt7 or Gria1. Neuronal subclass identity is shown by color-coding. (f-i) Correlation (f-h) and anti-correlation (i) of quantitative Kcnc1 expression and a subset of biophysical membrane parameters during the 1st AP.

Reference:

2Zeisel, A. et al. Brain structure. Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq. Science 347, 1138-1142 (2015).

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