Supplementary Figure 2: Iterative development of Patch-seq procedures. | Nature Biotechnology

Supplementary Figure 2: Iterative development of Patch-seq procedures.

From: Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes

Supplementary Figure 2

Step-wise improvements to the sampling, collection, ejection and analysis protocols are shown. Overall, 145 neurons were used to optimize recording and processing conditions, while ~120 neurons were processed to obtain reliable RNA-seq data. Axis labels on Bioanalyzer (Agilent) plots are: [FU], fluorescence unit; (bp) base pair. Magenta-colored “x” labels steps that had been omitted due to poorer outcomes.

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