Supplementary Figure 3: Quality control of cDNA libraries.
From: Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq
(a) Average size and concentration (from 300-9,000 bp) of all collected samples. Only samples with more than 200 pg/μl and an average size greater than 1,500 bp were sequenced (criteria denoted by dashed lines). Libraries generated from patched cells generally contained more (b) and higher quality, full-length (c) cDNA compared to negative controls in which no cell was patched. Distributions of concentrations (d) and average sizes (e) of cDNA libraries generated from ex vivo patched cells. Dashed lines in (b-e) indicate threshold criteria used for sequencing. (f) Pearson correlation between concentration of cDNA and number of genes detected for all sequenced ex vivo cells.
