Supplementary Figure 4: Mapping statistics of Patch-seq and standard Smart-seq2 libraries.
From: Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq
(a) Percentage of sequenced reads that aligned uniquely to the respective assembly for patched single neurons and dissociated HEK293T cells picked and sequenced according to standard Smart-seq2 protocol (Picelli et al. 2013). (b) Percentage of uniquely aligned reads that overlap annotated RefSeq exons, introns or between gene annotations. This comparison indicates that the libraries generated from patched single neurons have similar qualities as standard Smart-seq2 libraries generated from lysed whole cells.
