Supplementary Figure 1: Combinatorial synthesis of functionalized small molecules through the proposed orthogonal, iterative carbon-chain elongation platform using functionalized primers and extender units in combination with various termination pathways. | Nature Biotechnology

Supplementary Figure 1: Combinatorial synthesis of functionalized small molecules through the proposed orthogonal, iterative carbon-chain elongation platform using functionalized primers and extender units in combination with various termination pathways.

From: Energy- and carbon-efficient synthesis of functionalized small molecules in bacteria using non-decarboxylative Claisen condensation reactions

Supplementary Figure 1

Potential products are shown for 20 different combinations of ω or ω-1-functionalities from primers (R1) and α-functionalities from extender units (R2), listed in the first column, with varying β-functionalities from intermediate nodes with varying degrees of β-reductions (β-ketoacyl-CoA, β-hydroxyacyl-CoA, enoyl-CoA and acyl-CoA). In the structures of intermediate nodes and products, functional groups from primers (R1) are displayed in red and functional groups from extender units (R2) are displayed in blue. Products shown are produced through either acid-forming termination reactions (catalyzed by ACT) or alcohol-forming termination reactions (catalyzed by ACR+ADH). n represents the number of iterations/cycles of the platform, which determines the length of CoA-thioester intermediates and products. Check marks indicate the combinations of primers and extender units evaluated in this study.

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