Supplementary Figure 5: Time course of adipic acid production, glycerol consumption, acetate production and cell growth for a fermentation conducted in a controlled bioreactor. | Nature Biotechnology

Supplementary Figure 5: Time course of adipic acid production, glycerol consumption, acetate production and cell growth for a fermentation conducted in a controlled bioreactor.

From: Energy- and carbon-efficient synthesis of functionalized small molecules in bacteria using non-decarboxylative Claisen condensation reactions

Supplementary Figure 5

Strain MB263(DE3) ΔsucD overexpressing the engineered platform composed of PaaJ (thiolase), PaaH (HACDH), PaaF (ECH), TdTer (ECR), Acot8 (ACT) and Cat1 (activation enzyme) grown with glycerol as the sole carbon source (i.e. no succinic acid supplementation) with fermentation conditions as described in the Methods section. This profile corresponds to one of three independent (i.e. biological replicates) bioreactor fermentations.

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