Supplementary Figure 7: Stable transgenic plants carrying Rpi-amr3i under the regulation of a 35S promoter display resistance to all tested isolates
From: Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing
Transgenic diploid potato “Line 26” (Solynta B.V.) that express Rpi-amr3i under the 35S promoter are resistant to P. infestans isolates 88069 (upper), 06_3928A (middle) and EC3527 (bottom; right panel). The transgenic line displays no to weak HR at the place of inoculation. In contrast, transgenic plants carrying the non-functional candidate Rpi-amr3a (left panel) showed large necrotic lesions and sporulation. Each leaflet was inoculated with a droplet containing 500 spores; photographs were taken 6 dpi. Scale bar indicates 1cm. These experiments were repeated twice with similar results.
