Supplementary Figure 8: Relative levels of Rpi-amr3i expression under native regulatory elements in transgenic potato plants.
From: Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing
Relative copy number of Rpi-amr3i mRNAs per 1 million copies of EF1 mRNA internal control. Expression levels similar to wild-type Rpi-amr3i mRNA (lines 3-7) correspond to full resistance. Level of expression was measured in fully grown leaves from 10 and 14 week-old plants for Solynta transgenic and WT lines and in 12-week-old SP1102. Errors bars show standard deviation based on two time points. Primers show high specificity, as no amplification was observed in Solynta WT plants.
