Supplementary Figure 10: Rpi-amr3i establishes a new branch in the phylogeny of cloned functional Solanaceae R genes

(a) The Rpi-amr3 gene family forms a separate branch among previously cloned R genes. Gpa2/Rx represent the closest clade, members share however less than 35% amino-acid sequence identity. This maximum likelihood tree is based on the alignment of the full length amino-acid sequences of various functional Solanaceae NLR resistance genes, and Caenorhabditis elegans protein CED4 as outgroup. The numbers at nodes represent their bootstrap support (% support out of 100 bootstraps). (b) The phylogenetic tree of C18 cluster of R and S parents was constructed as described above, using all NLRs mapping to this cluster. C18 cluster members of resistant parent are named Rpi-amr3a-n, names of paralogs from susceptible parent start with NLR followed by number (see Supplementary File 7 for full NLR list).