Supplementary Figure 2: An example of chimeric MiSeq assembly of 2x250bp SP1102 RenSeq data
From: Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing
SPAdes assembled MiSeq sequence contigs were aligned to PacBio derived contigs with high stringency (Geneious). Top (red) and bottom (black) – PacBio contigs, middle – chimeric MiSeq contig with marked regions fitting PacBio contigs (black and red). Above and below SPAdes NODE_643 identity to PacBio Contig_105 and Contig_238 are shown, respectively. Grey area in SPAdes contig marks region of NB-ARC domain with 99% identity to both PacBio contigs (240nt), which is a part of larger fragment (650nt) displaying 96% identity between PacBio contigs. Overall identity between PacBio contigs (ORF region) is 85% on nucleotide level. ORFs are marked with arrows; NLR typical protein domains: coiled-coil (CC; purple box), nucleotide binding site (NB-ARC; blue box) and leucine-rich repeats (LRR; green box). Fused protein domain DUF 3542 is shown as light purple box. Drawn to scale.
