Supplementary Figure 4: Error rate of ROI reads and resulting assemblies.
From: Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing
(a) Box plot showing accuracy of ROI reads divided into bins based on their length (500 bp intervals). ROI reads that were used to de novo assemble the C18 clusters of resistant and susceptible parents, were re-aligned to the error corrected contigs. The percentage of accuracy was calculated for each individual ROI read based on the pairwise identity. (b) Bar graph showing total number of errors per ROI read coverage over de novo assembled contigs. WGS data was mapped to de novo assembled contigs of the C18 cluster and the mapping data was checked for errors. Colors represent the four bases.
